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sssi methylase  (New England Biolabs)


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  • 97

    Structured Review

    New England Biolabs sssi methylase
    Sssi Methylase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1069 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sssi+methylase/CpG+Methyltransferase/pmc12783656-193-6-9
    Average 97 stars, based on 1069 article reviews
    sssi methylase - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Methylation:

    Article Title: DNA methylation-mediated epigenetic regulation of oncogenic RPS2 as a novel therapeutic target and biomarker in hepatocellular carcinoma.
    Article Snippet: Ribosomal Protein S2 (RPS2) has emerged as a potential prognostic biomarker due to its involvement in key cellular processes and its altered expression pattern in certain types of cancer.. However, its role in hepatocellular carcinoma (HCC) has yet to be investigated.. Herein, we analyzed RPS2 mRNA expression and promoter methylation in HCC patient samples and HepG2 cells.

    Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways
    Article Snippet: .. The reference methylated DNA was prepared by methylation of the fully unmethylated DNA with SssI methylase (New England Biolabs, Cambridge, UK). .. Quantitative MSP was conducted with SYBR Green I (Roche) in the CFX Connect TM real-time PCR system (Bio-Rad Laboratories, CA, USA).

    Article Title: DNA methylation of the endothelin receptor B makes blue fish yellow
    Article Snippet: .. Plasmids were methylated in vitro using SssI methylase (#M0226L, New England Biolabs) for three hours, using S-adenosyl-methionine as a methyl donor twice during the reaction. ..

    Article Title: Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation
    Article Snippet: .. [ ] The unmethylated Klb promoter was obtained by transforming the luciferase reporter constructs into the dam‐/dcm‐ E. coli strain (New England Biolabs, Ipswich, MA), while the fully methylated Klb promoter was obtained by incubating the reporter constructs with the SssI methylase in the presence of S‐adenosylmethionine (New England Biolabs). .. The unmethylated or fully methylated Klb promoter reporter constructs were then transfected into Hepa 1–6 cells and the luciferase activity was measured using a Dual Luciferase Reporter Assay System (Promega, Madison, WI).

    Article Title: Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation.
    Article Snippet: .. [49] The unmethylated Klb promoter was obtained by transforming the luciferase reporter constructs into the dam-/dcm- E. coli strain (New England Biolabs, Ipswich, MA), while the fully methylated Klb promoter was obtained by incubating the reporter constructs with the SssI methylase in the presence of S-adenosylmethionine (New England Biolabs). .. The unmethylated or fully methylated Klb promoter reporter constructs were then transfected into Hepa 1–6 cells and the luciferase activity was measured using a Dual Luciferase Reporter Assay System (Promega, Madison, WI).

    Article Title: Epigenetics of Genes Preferentially Expressed in Dissimilar Cell Populations: Myoblasts and Cerebellum.
    Article Snippet: .. Methylation of the plasmids was targeted just to the ZNF556 inserts, which were the only CpG-containing sequences, by incubating the DNA construct (1 μg) with 4 units of SssI methylase and 160 μM S-adenosylmethionine (New England Biolabs) for 4 h at 37 ◦C or mock methylating by similar incubation but in the absence of S-adenosylmethionine. ..

    Article Title: Epigenetic Programming of Estrogen Receptor in Adipose Tissue by High Fat Diet Regulates Obesity-Induced Inflammation
    Article Snippet: .. The unmethylated promoter reporter was obtained by transforming the construct into dam – /dcm – E. coli strain (New England Biolabs), while the fully methylated reporter was obtained by treating the construct with SssI methylase (New England Biolabs) in the presence of S-adenosylmethionine. ..

    Control:

    Article Title: DNA methylation-mediated epigenetic regulation of oncogenic RPS2 as a novel therapeutic target and biomarker in hepatocellular carcinoma.
    Article Snippet: Ribosomal Protein S2 (RPS2) has emerged as a potential prognostic biomarker due to its involvement in key cellular processes and its altered expression pattern in certain types of cancer.. However, its role in hepatocellular carcinoma (HCC) has yet to be investigated.. Herein, we analyzed RPS2 mRNA expression and promoter methylation in HCC patient samples and HepG2 cells.

    Generated:

    Article Title: DNA methylation-mediated epigenetic regulation of oncogenic RPS2 as a novel therapeutic target and biomarker in hepatocellular carcinoma.
    Article Snippet: Ribosomal Protein S2 (RPS2) has emerged as a potential prognostic biomarker due to its involvement in key cellular processes and its altered expression pattern in certain types of cancer.. However, its role in hepatocellular carcinoma (HCC) has yet to be investigated.. Herein, we analyzed RPS2 mRNA expression and promoter methylation in HCC patient samples and HepG2 cells.

    Amplification:

    Article Title: DNA methylation-mediated epigenetic regulation of oncogenic RPS2 as a novel therapeutic target and biomarker in hepatocellular carcinoma.
    Article Snippet: Ribosomal Protein S2 (RPS2) has emerged as a potential prognostic biomarker due to its involvement in key cellular processes and its altered expression pattern in certain types of cancer.. However, its role in hepatocellular carcinoma (HCC) has yet to be investigated.. Herein, we analyzed RPS2 mRNA expression and promoter methylation in HCC patient samples and HepG2 cells.

    In Vitro:

    Article Title: DNA methylation of the endothelin receptor B makes blue fish yellow
    Article Snippet: .. Plasmids were methylated in vitro using SssI methylase (#M0226L, New England Biolabs) for three hours, using S-adenosyl-methionine as a methyl donor twice during the reaction. ..

    Luciferase:

    Article Title: Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation
    Article Snippet: .. [ ] The unmethylated Klb promoter was obtained by transforming the luciferase reporter constructs into the dam‐/dcm‐ E. coli strain (New England Biolabs, Ipswich, MA), while the fully methylated Klb promoter was obtained by incubating the reporter constructs with the SssI methylase in the presence of S‐adenosylmethionine (New England Biolabs). .. The unmethylated or fully methylated Klb promoter reporter constructs were then transfected into Hepa 1–6 cells and the luciferase activity was measured using a Dual Luciferase Reporter Assay System (Promega, Madison, WI).

    Article Title: Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation.
    Article Snippet: .. [49] The unmethylated Klb promoter was obtained by transforming the luciferase reporter constructs into the dam-/dcm- E. coli strain (New England Biolabs, Ipswich, MA), while the fully methylated Klb promoter was obtained by incubating the reporter constructs with the SssI methylase in the presence of S-adenosylmethionine (New England Biolabs). .. The unmethylated or fully methylated Klb promoter reporter constructs were then transfected into Hepa 1–6 cells and the luciferase activity was measured using a Dual Luciferase Reporter Assay System (Promega, Madison, WI).

    Construct:

    Article Title: Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation
    Article Snippet: .. [ ] The unmethylated Klb promoter was obtained by transforming the luciferase reporter constructs into the dam‐/dcm‐ E. coli strain (New England Biolabs, Ipswich, MA), while the fully methylated Klb promoter was obtained by incubating the reporter constructs with the SssI methylase in the presence of S‐adenosylmethionine (New England Biolabs). .. The unmethylated or fully methylated Klb promoter reporter constructs were then transfected into Hepa 1–6 cells and the luciferase activity was measured using a Dual Luciferase Reporter Assay System (Promega, Madison, WI).

    Article Title: Epigenetic Regulation of Hepatic Lipid Metabolism by DNA Methylation.
    Article Snippet: .. [49] The unmethylated Klb promoter was obtained by transforming the luciferase reporter constructs into the dam-/dcm- E. coli strain (New England Biolabs, Ipswich, MA), while the fully methylated Klb promoter was obtained by incubating the reporter constructs with the SssI methylase in the presence of S-adenosylmethionine (New England Biolabs). .. The unmethylated or fully methylated Klb promoter reporter constructs were then transfected into Hepa 1–6 cells and the luciferase activity was measured using a Dual Luciferase Reporter Assay System (Promega, Madison, WI).

    Article Title: Epigenetics of Genes Preferentially Expressed in Dissimilar Cell Populations: Myoblasts and Cerebellum.
    Article Snippet: .. Methylation of the plasmids was targeted just to the ZNF556 inserts, which were the only CpG-containing sequences, by incubating the DNA construct (1 μg) with 4 units of SssI methylase and 160 μM S-adenosylmethionine (New England Biolabs) for 4 h at 37 ◦C or mock methylating by similar incubation but in the absence of S-adenosylmethionine. ..

    Article Title: Epigenetic Programming of Estrogen Receptor in Adipose Tissue by High Fat Diet Regulates Obesity-Induced Inflammation
    Article Snippet: .. The unmethylated promoter reporter was obtained by transforming the construct into dam – /dcm – E. coli strain (New England Biolabs), while the fully methylated reporter was obtained by treating the construct with SssI methylase (New England Biolabs) in the presence of S-adenosylmethionine. ..

    Incubation:

    Article Title: Epigenetics of Genes Preferentially Expressed in Dissimilar Cell Populations: Myoblasts and Cerebellum.
    Article Snippet: .. Methylation of the plasmids was targeted just to the ZNF556 inserts, which were the only CpG-containing sequences, by incubating the DNA construct (1 μg) with 4 units of SssI methylase and 160 μM S-adenosylmethionine (New England Biolabs) for 4 h at 37 ◦C or mock methylating by similar incubation but in the absence of S-adenosylmethionine. ..



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